Genomic DNA
Purified plant DNA with concentration and purity values.
Useful forPCR, genotyping and sequencing workBiological
Plant Tissue Culture
Molecular testing and culture support for plant material
Molecular and biochemical testing of plant material — DNA extraction, PCR with gel electrophoresis and GM or marker screening — plus expert advice on plant tissue culture work.
6 test optionsFrom leaf tissue to gel resultGM and marker gene screeningTypical turnaround 5–8 working days
Plant Tissue Culture processIllustrative
01 / Overview
What is Plant Tissue Culture?
Genomic DNA is extracted from leaf, callus or seed tissue and checked for quantity and purity. A chosen DNA sequence — a transgene, a marker gene or a GM element — is then amplified by PCR, and the products are separated on an agarose gel, where a band of the expected size shows the target is present.
Because PCR detects the sequence itself, transformation or GM status can be confirmed from a small piece of tissue, long before plants mature. Consultation support covers media, sterilisation, explant choice and regeneration for tissue culture work.
02 / How it works
How it works
- 01
Tissue is sampled
Leaf, callus, seed or plantlet tissue is collected and sent fresh, frozen or dried.
- 02
DNA is extracted
Cells are broken open and genomic DNA is purified, then checked for quantity and purity.
- 03
Target is amplified
Primers for the gene, marker or GM element are copied over repeated heating and cooling cycles.
- 04
Bands are read
Products run on an agarose gel; a band of the expected size against a ladder confirms the target.
03 / What it measures
What it measures
Transgene & marker confirmation
Confirm that a gene or selectable marker is present in each plant.
Useful forTransformed lines, tissue-culture regenerantsGM screening
Detect GM-specific DNA elements in plants, seeds or grain.
Useful forSeed lots, food and feed materialUrease activity
Measure urease enzyme activity in plant material or extracts.
Useful forSoybean products, enzyme studiesTissue culture guidance
Advice on media, sterilisation, explants and regeneration.
Useful forMicropropagation and callus culture set-up04 / Test options
Choose the Plant Tissue Culture options you need
6 options · none added yet
05 / Sample requirements
Sample requirements
- Accepted forms
- Fresh or frozen leaf, callus, plantlets; seeds or grain; leaf dried on silica gel
- Quantity
- Around 100–500 mg tissue per sample; a few grams for seed or grain
- Transport
- Chilled or frozen, sealed and individually bagged, within 48 h
- Primers & controls
- Primer sequences, expected band size and a positive control if available
- Young, healthy, disease-free tissue
- Each sample bagged and labelled separately
- Share primer details and the expected band size
- No rotten, mouldy or contaminated material
- No live regulated or GM material without the required permits
Hazardous or air-sensitive samples: mention it in your request, and attach the MSDS if you have one.
06 / Results & turnaround
Results & turnaround
What you receive
- Agarose gel images with DNA ladder
- Present / absent call for each target
- DNA concentration and purity values
- Urease activity values where requested
- Report PDF
Turnaround & pricing
Typically 5–8 working days after samples reach the lab.
Price confirmed in your quotation, depending on user type, options and number of samples.
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