Chromatography & Separation

Ion Chromatography

Separation and quantification of anions and cations

Ion chromatography separates and quantifies dissolved ions such as chloride, nitrate, sulfate, sodium and ammonium. It is a standard method for common anions and cations in water and aqueous extracts.

1 test optionCommon anions & cationsSub-mg/L detection limitsTypical turnaround 4–6 working days
Ion Chromatography measurement principleIllustrative
EluentPumpInjectorColumnSuppressorConductivity cellChromatogram
Ions, held back and releasedIllustrative
01 / Overview

What is Ion Chromatography?

In ion chromatography an eluent carries the sample through an ion-exchange column, where ions are held back to different extents and leave at different times. A suppressor lowers the background conductivity of the eluent, and a conductivity detector measures each ion as it elutes.

IC quantifies several ions in one injection, including species such as nitrate, sulfate, phosphate and ammonium that elemental methods cannot tell apart. For total metal content, ICP-OES or ICP-MS is usually the better choice.

02 / How it works

How it works

  1. 01

    Sample is filtered

    Aqueous samples are filtered and diluted into the working range.

  2. 02

    Ions are separated

    Eluent carries the sample through an ion-exchange column; each ion leaves at its own time.

  3. 03

    Background is suppressed

    A suppressor exchanges eluent ions so analyte ions stand out in conductivity.

  4. 04

    Peaks give concentrations

    Peak areas are compared with standards to give each ion in mg/L.

03 / What it measures

What it measures

Common anions

Fluoride, chloride, nitrate, sulfate, phosphate and more.

Useful forDrinking water, groundwater, effluents

Common cations

Sodium, potassium, ammonium, calcium and magnesium.

Useful forWater, soil extracts, fertilisers

Nutrients in water

Nitrate, nitrite, phosphate and ammonium levels.

Useful forEnvironmental and agricultural water

Ionic impurities

Trace ions in chemicals, process water and extracts.

Useful forChemicals, pharma, high-purity water

Organic acid anions

Acetate, formate, oxalate and similar anions.

Useful forFermentation broths, foods, process liquors
04 / Test options

Choose the Ion Chromatography options you need

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Ion Chromatography test options
05 / Sample requirements

Sample requirements

Accepted forms
Water, effluents and aqueous extracts; solids after leaching, by discussion
Quantity
20–50 mL of liquid per sample
Container
Clean plastic (PP or HDPE) bottles with tight caps
Ions
List the anions or cations needed and their expected range
Storage
Keep cool (around 4 °C); nitrite and phosphate should be analysed promptly
  • Clean plastic bottles, filled and tightly capped
  • Keep samples chilled and send them promptly
  • Mention high salt, acid or organic content in the matrix
  • No acid or chemical preservatives unless the lab advises
  • No oily or particle-laden samples without discussing first

Hazardous or air-sensitive samples: mention it in your request, and attach the MSDS if you have one.

06 / Results & turnaround

Results & turnaround

What you receive

  • Chromatogram for each sample
  • Concentration of each requested ion (mg/L)
  • Retention times and peak areas
  • Calibration and check-standard details
  • Report PDF

Turnaround & pricing

Typically 4–6 working days after samples reach the lab.

Price confirmed in your quotation, depending on user type, options and number of samples.

Sample report · IllustrativeExample chloride42.6 mg/LIllustrative — not measured sample data
07 / Limitations

When Ion Chromatography isn't the right fit

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