Microscopy & Imaging

TEM

Transmission Electron Microscopy

TEM passes a high-energy electron beam through an ultra-thin sample to image its internal structure. It shows nanoparticle size and shape, core–shell layers and crystal defects, and with EDX adds elemental composition.

3 test optionsSub-nanometre resolutionSamples thinner than ~100 nmTypical turnaround 7–10 working days
TEM measurement principleIllustrative
Electron gunCondenser lensThin sampleObjective lensProjector lensScreen / cameraTEM image
Electrons that pass right throughIllustrative
01 / Overview

What is TEM?

Transmission Electron Microscopy accelerates electrons, typically at 80–200 kV, and passes them through a sample thin enough to be electron-transparent. Magnetic lenses magnify the transmitted beam onto a screen or camera; thicker, denser or heavier regions scatter more electrons and appear darker.

Because the image is formed by electrons that pass through the sample, TEM shows what lies inside a particle — cores, shells, pores and defects — at far higher resolution than SEM. Powders are simply dispersed on a carbon-coated grid; bulk materials must be thinned first.

02 / How it works

How it works

  1. 01

    Sample on a grid

    Powders are dispersed in a solvent, dropped onto a carbon-coated copper grid and dried.

  2. 02

    Beam passes through

    In high vacuum, electrons pass through the thin sample; denser regions scatter more of them.

  3. 03

    Lenses magnify

    Objective and projector lenses magnify the transmitted beam many thousands of times.

  4. 04

    Image is recorded

    A camera records the magnified image as a bright-field micrograph with a scale bar.

03 / What it measures

What it measures

Particle size & shape

Measure individual nanoparticles directly from the images.

Useful forNanoparticles, quantum dots, colloids

Internal structure

See core–shell layers, hollow particles and pores inside the sample.

Useful forCore–shell particles, capsules, porous materials

Dispersion & agglomeration

Check whether particles sit apart or cluster together.

Useful forNanocomposites, suspensions, catalysts

Crystallinity & defects

See grains, dislocations and, at high magnification, lattice fringes.

Useful forMetals, thin films, crystalline nanoparticles

Elemental composition (EDX)

Identify the elements in a particle or region and their approximate amounts.

Useful forAlloys, doped and mixed nanoparticles

Elemental maps

Show where each element sits across the imaged area.

Useful forCore–shell, dopant and segregation studies
04 / Test options

Choose the TEM options you need

3 options · none added yet

TEM test options
05 / Sample requirements

Sample requirements

Accepted forms
Nanopowders, colloidal dispersions, ready-made grids or thin sections
Quantity
About 5–10 mg of powder or 1 mL of dispersion
Thickness
Under ~100 nm at the area imaged; bulk solids must be thinned first
Dispersion
Tell us a solvent the powder disperses in (e.g. ethanol, water)
Condition
Dry, vacuum-stable and stable under the electron beam where possible
  • Dry powders in sealed, labelled vials
  • Name a solvent the sample disperses in
  • Declare magnetic or beam-sensitive samples
  • No bulk pieces unless already thinned to under ~100 nm
  • No samples that outgas or decompose in vacuum

Hazardous or air-sensitive samples: mention it in your request, and attach the MSDS if you have one.

06 / Results & turnaround

Results & turnaround

What you receive

  • TEM micrographs at several magnifications, with scale bars
  • Particle size measurements on request
  • EDX spectra and composition tables (if selected)
  • Elemental maps (if selected)
  • Raw image files
  • Report PDF

Turnaround & pricing

Typically 7–10 working days after samples reach the lab.

Price confirmed in your quotation, depending on user type, options and number of samples.

Sample report · IllustrativeExample mean particle size18 nmIllustrative — not measured sample data
07 / Limitations

When TEM isn't the right fit

Let’s find your next step

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